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pet21 vector  (New England Biolabs)


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    New England Biolabs pet21 vector
    Pet21 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet21+vector/bio_rxiv__2024__05__07__592971-142-17-22?v=New+England+Biolabs
    Average 86 stars, based on 1 article reviews
    pet21 vector - by Bioz Stars, 2026-07
    86/100 stars

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    A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

    Journal: bioRxiv

    Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

    doi: 10.1101/2024.12.02.626473

    Figure Lengend Snippet: A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

    Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

    Techniques: Incubation

    SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

    Journal: bioRxiv

    Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

    doi: 10.1101/2024.12.02.626473

    Figure Lengend Snippet: SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

    Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

    Techniques: SDS Page, Construct, Plasmid Preparation, Positive Control, Marker, Control

    Journal: bioRxiv

    Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

    doi: 10.1101/2024.12.02.626473

    Figure Lengend Snippet:

    Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

    Techniques: